Biology

Last Updated on November 15, 2022 by Mostafa Try to mention the expression of genetic engineering in humans among a bunch of people, or a community gathering. Now try to escape the endless amount of questions, hurdles, and debates that will arise. technical  Since their first days, genetic engineering, gene modification, and traits selection have always been controversial and have …

Last Updated on July 30, 2022 by Mostafa ELISpot assay stands for the enzyme-linked immunospot assay. It is a very sensitive method used for measuring the number of cytokines secreting cells. And then measuring the antigen-specific T cells. In this article we will disscus ElISpot principle, steps, benifits, protocol, applications and more.   ELISpot Assay for T Cell Responses ELISpot …

Last Updated on December 12, 2022 by Nourhan Essam The Enzyme Linked Immunosorbent Assay (ELISA) is one of the most sensitive immunoassays available. The typical detection range for an ELISA is 0.1 to 1 fmole or 0.01 ng to 0.1 ng. It is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones.  In an ELISA, …

Last Updated on September 20, 2022 by Mostafa Western blot is an indispensable mechanism in the modern biomedical research laboratory, as well as in laboratories doing research in other areas. It is considered as an analytical technique used mainly in molecular biology and immunogenetics where antibodies are used to specifically detect their antigen. Before discussing how to analyze western blot …

Last Updated on August 1, 2022 by Sherouk Badr Shehata Before talking about cell cycle regulation, we should know more about the term “Cell Cycle” and what it means. Definition of Cell Cycle Cell cycle is the process through which cells replicate and make two new cells. We can also define cell cycle as a series of events that takes …